The aim of this study was to investigate the relationship between different personality variables and pathological gambling (PG). The NEO-FFI and measures of impulsivity and sensation-seeking were administered to a sample of pathological gamblers (n = 90) and to a contrast group of non-pathological gamblers (n = 66) matched on sex and age. Gender, age, education level and the personality variables were entered into crude and adjusted logistic regression analyses with PG-status as the dependent variable. The results showed that educational level and all personality variables were significant predictors of PG in the crude analyses, however only four of the 12 significant predictor variables (Neuroticism, Openness, Impulsivity, and need for Stimulus Intensity) remained significant in the adjusted analysis. All predictor variables accounted for 71% of the variance in PG-status. Clinical implications of the findings are discussed.